Since the success of the artificial breeding of Obsidian Oriental Fish Farm in 1991, the country has had the capacity to produce nearly a million larvae in 2000, which will play a very important role in promoting the aquaculture production of Obsidus orientalis. However, due to a variety of factors such as the overfishing of broodstock and water pollution, the resources of the dark-skinned oriental fish in the Yangtze River tend to be depleted. Therefore, artificial propagation of the artificial broodstock of the dark-skinned oriental fish is an urgent task. The key technologies are introduced as follows:
First, broodstock selection should be selected as a strong physique, desalination is full, gonads mature broodstock as a reserve broodstock. Females weigh more than 500g, males more than 400g, and male and female ratios of 1.5:1 are preferred.
Second, the broodstock cultivation The use of artificial seawater cultivation of dark-pattern oriental fish brood broodstock artificial reproduction, the general pool water depth of 0.8m, broodstock stocking density l-2 tail / square meter. The use of circulating filtration and vortex air pump oxygenation measures require the dissolved oxygen in the water to be above 5mg/L. The pool water temperature should not be too high, preferably 17°C to 20°C. The bait may be fed with squid bait, and the mixed fish may be properly mixed. Shrimp. Set up a bait station and feed it once a day in the afternoon and afternoon, and the amount of bait should be taken until the evening of eating.
Third, oxytocin incubation After culturing the broodstock, depending on the degree of gonadal development, it was decided to inject different doses of oxytocin. In general, artificially synthesized release hormone (LRH-A) and salmon pituitary (PG) were injected in a mixed manner. LRH-A50ug and PG 4-6mg were injected per kg of fish. After the oxytocin has been injected, the broodstock is returned to the holding tank and the situation is observed regularly. After hormone production, the effect time is generally 21-45 hours.
The hatching methods of fertilized eggs mainly include hatching bucket water hatching and sticky egg hatching hydrostatic hatching. During the entire incubation process, the temperature is controlled at 18-22°C. The amount of dissolved oxygen in water is more than 6mg/L. Under normal circumstances, it takes about 130 hours for a broodstock to grow from a fertilized egg to hatch a larvae.
Fourth, fingerlings are generally cultivated in fish ponds with an area of ​​2,000 to 3,300 square meters and a water depth of more than 1.5 meters. The stocking density is 2-3 tails per square meter. The newly hatched larvae can feed after 3-4 days. The open bait is a relatively small rotifer. Afterwards, with the growth of the individual, the bait begins to transition from small clumps, large horns, and burned feet. Small fish etc. The amount of fish fed every day accounts for 3% to 5% of body weight, so it is advisable to eat it before the evening.
5. Overwintering Management Because the dark-faced oriental fish is not tolerant of low temperatures, the artificial winter temperature of the fish species is preferably controlled at about 14°C. The wintering density is 5-7 kg/m3. When the water temperature is above 10°C, feeding is required, the water exchange rate should reach 100%, and the air should be continuously inflated. When the water temperature is below 10°C, the fingerlings will stop feeding and the water change amount can be reduced to 30% to 40%. Discontinuously inflated and inflated for 30 minutes each time. , stop for 30 minutes.
Sixth, disease prevention fish species into the pool with 5mg / L furazolidone Quanchiposa to remove body surface parasites. When feeding bait, vitamin E, C, etc. should be added to prevent fishery nutrient diseases. During the winter, antibiotics can be used to disinfect the body surface every 10-15 days. The disease prevention effect is good.
First, broodstock selection should be selected as a strong physique, desalination is full, gonads mature broodstock as a reserve broodstock. Females weigh more than 500g, males more than 400g, and male and female ratios of 1.5:1 are preferred.
Second, the broodstock cultivation The use of artificial seawater cultivation of dark-pattern oriental fish brood broodstock artificial reproduction, the general pool water depth of 0.8m, broodstock stocking density l-2 tail / square meter. The use of circulating filtration and vortex air pump oxygenation measures require the dissolved oxygen in the water to be above 5mg/L. The pool water temperature should not be too high, preferably 17°C to 20°C. The bait may be fed with squid bait, and the mixed fish may be properly mixed. Shrimp. Set up a bait station and feed it once a day in the afternoon and afternoon, and the amount of bait should be taken until the evening of eating.
Third, oxytocin incubation After culturing the broodstock, depending on the degree of gonadal development, it was decided to inject different doses of oxytocin. In general, artificially synthesized release hormone (LRH-A) and salmon pituitary (PG) were injected in a mixed manner. LRH-A50ug and PG 4-6mg were injected per kg of fish. After the oxytocin has been injected, the broodstock is returned to the holding tank and the situation is observed regularly. After hormone production, the effect time is generally 21-45 hours.
The hatching methods of fertilized eggs mainly include hatching bucket water hatching and sticky egg hatching hydrostatic hatching. During the entire incubation process, the temperature is controlled at 18-22°C. The amount of dissolved oxygen in water is more than 6mg/L. Under normal circumstances, it takes about 130 hours for a broodstock to grow from a fertilized egg to hatch a larvae.
Fourth, fingerlings are generally cultivated in fish ponds with an area of ​​2,000 to 3,300 square meters and a water depth of more than 1.5 meters. The stocking density is 2-3 tails per square meter. The newly hatched larvae can feed after 3-4 days. The open bait is a relatively small rotifer. Afterwards, with the growth of the individual, the bait begins to transition from small clumps, large horns, and burned feet. Small fish etc. The amount of fish fed every day accounts for 3% to 5% of body weight, so it is advisable to eat it before the evening.
5. Overwintering Management Because the dark-faced oriental fish is not tolerant of low temperatures, the artificial winter temperature of the fish species is preferably controlled at about 14°C. The wintering density is 5-7 kg/m3. When the water temperature is above 10°C, feeding is required, the water exchange rate should reach 100%, and the air should be continuously inflated. When the water temperature is below 10°C, the fingerlings will stop feeding and the water change amount can be reduced to 30% to 40%. Discontinuously inflated and inflated for 30 minutes each time. , stop for 30 minutes.
Sixth, disease prevention fish species into the pool with 5mg / L furazolidone Quanchiposa to remove body surface parasites. When feeding bait, vitamin E, C, etc. should be added to prevent fishery nutrient diseases. During the winter, antibiotics can be used to disinfect the body surface every 10-15 days. The disease prevention effect is good.
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