1. Abnormality examination: Take a drop of semen onto a glass slide, naturally dry, rinse with 1% alcohol for 1-2 minutes, rinse with 0.5% gentian violet or red-blue ink for 3 minutes, dry after microscopic examination, 300 —How many spermatozoa are there in 500 sperm?
2. Appearance check: normal semen is milky opaque liquid. . The blood was mixed pink; was faeces contaminated yellow-brown; the urate was mixed pinkish-white cotton-like; excess transparent liquid mixed with water-like; where the quality of contaminated semen dropped sharply, the fertilization rate will not be high.
3, density check:
Estimation method: 1 Density: under the microscope, the entire field is covered with sperm, almost no void, more than 4 billion / ml; 2 medium density: obvious sperm distance, 2-4 billion / ml; 3 density thin: there is a lot of sperm Void, less than 2 billion / ml.
4. Vitality check: After 20 to 30 minutes after sperm sampling, take one drop of each of semen and normal saline, mix on the end of the slide, and place a cover glass. It is not easy for semen to be too much, and it is advisable to fill two gaps without overflowing. In 200-400 times microscope at 37 °C; straight forward movement, with fertilization ability, accounting for the proportion of how to score 0.1-0.9. There is no fertilization ability in both circular motion and swing. High-energy, high-density semen swirls.
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