Rat (5-NT) ELISA kit technology principle
Rat 5 Nuclease (5-NT) ELISA Kit Rat 5 Nuclease (5-NT) ELISA Kit Technology Supports Rat 5 Nucleotide (5-NT) ELISA Kit Instructions for Shanghai Qiao Yu Biotechnology Co., Ltd. specializes in providing various species, various series of ELISA kit test kits, ELISA kit technical support, ELISA kit technology principles, ELISA assay kit instructions. All of the Elisa kits of Qiao Yu provide free testing services, providing complete technical guidance from the whole process of specimen collection, preservation, pre-test, experiment, data analysis, etc., specializing in imported sub-packages and original and domestic Elisa kits. Quality Assurance! Welcome new and old customers to call to order!
Product Name: Rat (5-NT) ELISA kit technology principle
English name: Rat 5-Nucleotidase, 5-NT ELISA Kit
Product No:QY-SZ2075
Product traits: liquid
Product use: dedicated to scientific research
Product specifications: 96T/48T
Customer Service Phone: 021-51867124 QQ Email @qq.com
Experimental principle
The microplate is coated with the purified antibody to prepare a solid phase carrier, and a sample or a standard, a biotinylated anti-antibody antibody, and an HRP-labeled avidin are sequentially added to the micropore coated with the antibody against the index. After thorough washing, the substrate was developed with TMB. TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with this indicator in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration.
Kit composition:
Sealing film: 2 pieces (48) / 2 pieces (96)
Instructions: 1 copy
Sealed bag: 1
Standard: 2700ng/L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation
Enzyme label coated plate: 1×481×962-8°C
Sample dilution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C preservation
Reagent A: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C preservation
Developer B solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C preservation
Stop solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C preservation
Concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation
Collection and preservation of specimens
1. Serum: Whole blood samples should be placed at room temperature for 2 hours or 4 °C overnight, centrifuged at 1000 xg for 20 minutes, the supernatant can be detected, or the specimens should be stored at -20 ° C or -80 ° C, but should be avoided. Freezing and thawing.
2. Plasma: EDTA or heparin can be used as anticoagulant. Centrifuge at 2 - 8 ° C 1000 xg for 15 minutes within 30 minutes after collection, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing. melt.
3. Cell culture supernatant or other biological specimens: centrifuge at 1000 xg for 20 minutes, take the supernatant for testing, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing and thawing.
Note: Specimen hemolysis will affect the final test results, so hemolysis specimens should not be tested.
4. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When the components in the cells were detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
5. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. A certain amount of PBS (pH 7.4) was added, and the specimen was homogenized by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use.
6. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.
7. Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
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